Graduate Student Florida A&M University Tallahassee, Florida
Several cannabinoids and terpenes such as cannabidiol, cannabichromene, cannabidiolic acid, cannabidivarin, cannabinol, cannabigerol and β-caryophyllene, bisabolol, myrcene, linalool, limonene, geraniol, neralidol, α-pinene, α-terpineol, γ-terpinene respectively were screened against MDA MB 231 Doxorubicin RT, H1975 Osimertinib RT, PDX Lung cancer cell lines using 2D cytotoxicity and 3D spheroids assay. The cytotoxic potential of combinations of cannabinoids with β-caryophyllene increased by 10 folds in comparison to their individual anticancer activity. The combination index values were < 0.5 suggesting synergism. Colony formation assay showed reduction in number of colonies by 10 folds in individual treatments and 20 folds in treatment with combinations (P < 0.0001). Wound healing and apoptosis assays exhibited similar pattern (P < 0.001). Cell cycle analysis by flow cytometry demonstrated large number of cells in G1 phase with combinations relative to individual compounds emphasizing cell cycle arrest. Most potent combinations from in-vitro were cannabichromene and cannabidiolic acid with β-caryophyllene which will be further evaluated in-vivo.
Learning Objectives:
The objective of this study was to find an effective combination of two drugs that can overcome cancer resistance.
The effect of combinations of various cannabinoids and terpenes was studied.
Screen several cannabinoids, terpenes and their combinations against resistant cancer cell lines to get the most potential combination.