【Objective】 We have developed ultrasound-responsive nanobubbles (NBs) as gene delivery tools and echo-contrast agents. In this study, to establish an efficient delivery system for nucleic acid, we prepared miRNA-loaded NBs (mi-NBs) by coating anionic NBs with cationic polysaccharides. The combination of mi-NBs and ultrasound was assessed for the suppression of tumor. 【Methods】 Methyl glycol chitosan (MGC) was added to anionic NBs to prepare MGC-coated NBs (MGC-NBs). The capability of MGC-NBs for ultrasound imaging, miRNA loading, and intracellular delivery were investigated. In addition, a tumor-suppressive miRNA, miR-145, was transfected to tumor-bearing mice by mi-NBs and ultrasound, and their effects on tumor growth were evaluated. 【Conclusions】MGC-NBs possessed the ability to load miRNAs and to deliver miRNA by the combination with ultrasound. Furthermore, the transfection of miR-145 allowed for a tumor growth inhibitory in vivo. These results indicate that mi-NBs may be a useful systemic delivery tool for nucleic acid.
Learning Objectives:
Upon completion, participant will be able to demonstrate the miRNA delivery to tumors by ultrasound-responsive carriers.
Upon completion, participant will be able to describe various nucleic acid delivery systems.
Upon completion, participant will be able to understand miR-145 delivery is related to triple negative breast cancer depress.