Graduate Student University of Missouri-Kansas City Groton, Connecticut
Despite the increased application of 3D-printed devices, there is no necessary information about their relevant toxicity. The biocompatibility of 3D-printed stents could depend on the concentration of the active material, physical properties, and the duration of exposure. To evaluate the dose-dependent toxicity, zebrafish embryos were incubated with 1 mM and 2 mM of resveratrol (RSL) in 3D-printed material (4 mm diameter and thickness of 1 mm). It was found that stent with 1 mM dose indicated no mortality during the developmental stages. However, stent with 2 mM dose stent demonstrated reduced survival rate of 93.3±3.3 %. At 72 hpf several developmental abnormalities were noticed in embryos treated with the stent loaded with 2 mM dose. The results demonstrate the RSL-loaded stents did not exert any inflammatory responses in macrophages. The RSL-eluting 3D-printed stent could down-regulate the proinflammatory cytokines and H2O2-induced developmental abnormalities in the embryos.
Learning Objectives:
Upon completion, participant will gain insights into assessing the biocompatibility of 3D-printed materials using zebrafish embryo toxicity studies.
Upon completion, participant will able to understand the protocol for developing the acute inflammatory model in the zebrafish embryos.
Upon completion, participant will able to learn the protective action of resveratrol against hydrogen perioxide induced developmental defects